Kaboğlu, Cem Buğra (2016) Comparison of Dermal Tissue Derived Mesenchymal Stem Cells and Dermal Tissue Derived Epithelium Cells in-vitro Wound Healing Model In Terms of Migration Ability and Speed. Other thesis, TED Ankara Koleji.
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Abstract
In nowadays an increase of wars or accidents around the globe can be observed. Those happenings cause individuals to expose burns, radiation burns or acute wounds. Today those wounds are treated with a method called ‘’skin grafting’’ which is known to lower the patients’ quality of life and costs thousands of dollars. The aim of this research is to find an alternative treatment to dermal wounds and decide which of the two cell types –Mesenchymal Stem Cell (MSCs) and Epithelium Cells (ECs)- would suitable to be used in this alternate method. My research question is ‘’ How do dermal tissue derived Mesenchymal Stem Cells and dermal tissue derived Epithelium Cells affect the wound healing process in terms of covering the wound area, measured the cell index and percentage wound area coverage by using Real Time Cell Analyzer Migration Experiment and in-vitro wound healing model ?’’ The methods used to investigate the research question are Real Time Cell Analyzer Migration Experiment and in-vitro wound healing model. Before the both experiments flow-cytometry is done on cells on the third passage in order to identify them as MSCs derived tissues, with respect to surface markers CD11b/c, CD45, CD90, CD49. After the Real Time Cell Analyzer Migration experiment 48 hour mean values of cell indexes for the cells are as follows; 0.82 for MSCs (from 0.0815 to 0.3451) and 0.71 for ECs (from 0.0819 to 0.2019). In in-vitro model it was observed MSCs covered 100 % of the wound gap at 10. Hour, ECs covered 17% of the area at 48. Hour which showed a meaningful difference ( p˂ 0.05) in t-test. The conclusion is that MSCs could be used as a cell spray rather than ECs for a batter treatment to dermal wounds or burns. This study merits further investigation for usage of MSCs in wound healing model as a spray.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | Supervisor: Demet İzgü |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Users 114 not found. |
| Date Deposited: | 21 Sep 2016 07:47 |
| Last Modified: | 21 Sep 2016 08:59 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/744 |
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