Sevil, Zekiye Elvin (2015) Investigating the effects of changing concentrations of Hydrogen Peroxide on DNAs of lentil (Lens culnaris) and chickpea (Cicer arietinum)plants. Other thesis, TED Ankara Koleji.
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Abstract
Strong chemical agents have been a part of our lives for quite some time; they have made many contributions in various fields, and are still being used frequently. However such common chemicals, like the ones that are being used in our houses for cleaning, can also bring harm to us and our environment. Aside from being used in industrial and domestic fields, such chemicals are also used in agricultural fields as, for example, pesticides. It is possible for these pesticides to harm plants, physically and even have mutagenic effects on it. Considering these factors, this study concentrated on Hydrogen Peroxide (H2O2) which can be used in pesticides along with its usage as cleansing agent. Due to its nature, it is highly possible for H2O2 to bring harm to plant phsically and even affect its DNA, when present in excessive concentrations. The aim of this study was to investigate what sort of effect does different concentrations of H2O2 have on different plants, that being Lens culnaris and Cicer arietinum. It was found that as H2O2 concentration was increased, damage done on plant DNA also increased, which was examined by the comet like formation of damaged DNA fragments. As the concentration increased, more fragments from the cell nucleus shattered and the comet got longer. The maximum amount of damage was done by the highest concentration 36%, after 15 minutes of exposure to H2O2. Overall the data obtained from the examination of comets support the hypothesis that H2O2 would have a similar harmful effect on the plants, regardless of their species.
| Item Type: | Thesis (Other) |
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| Additional Information: | Supervisor: Fuat İsmet ŞİŞMAN, IB Notu: C |
| Uncontrolled Keywords: | DNA Comet Assay Method, Hydrogen Peroxide (H2O2), lentil (Lens culnaris), chickpea (Cicer arietinum), pesticide |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 27 Aug 2015 10:27 |
| Last Modified: | 27 Aug 2015 10:27 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/701 |
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