Özcebe, Zeynep Çağla (2010) Comparison of mutagenic effects of regular and environment friendly detergents on the Ames Salmonella/microsome mutagenicity assay. Other thesis, TED ANKARA COLLEGE FOUNDATION HIGH SCHOOL.
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Abstract
The aim of this extended essay is to investigate the mutagenic effects of two different detergents (one is known to be environment friendly) by using a well standardized screening test; The Ames Salmonella/microsome mutagenicity assay. The research question was “Is there a significant difference between regular detergent and environment friendly detergent in terms of promoting mutations in the Ames Salmonella/microsome mutagenicity assay?” It was hypothesized that; there would be a significant mean difference in terms of promoting mutations between the groups. The regular detergent will show more mutagenic effect, followed by environment friendly detergent. The Ames Salmonella/microsome mutagenicity plate incorporation assay was used for testing this hypothesis. In the first stage, toxicity assay was performed. Different dilutions of both detergents (1x10‐2, 1x10‐3 and 1x10‐4) were tested in the overnight Salmonella nutrient broth cultures. The Salmonella strain TA100 and, an enriched, non‐selective agar medium was used in toxicity assay. The tested dilutions of two detergents were found to be non‐toxic for tested strain. If planned testing dilutions of these detergents were found to be toxic for Salmonella strain TA100, it could not be possible to perform mutagenicity assay. On the second stage of assay, these dilutions of two different detergents were tested according to the procedure defined in The Ames Salmonella/microsome mutagenicity plate incorporation assay using TA98 and TA100 Salmonella strains. The ANOVA results revealed that there were no statistically significant difference between two detergents and between each detergent and negative control group in terms of mutagenicity. This finding was not supporting the hypothesis that environment friendly detergent would induce less mutagenic effects compared to regular detergent.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | Supervisor: Meltem Başer |
| Uncontrolled Keywords: | mutagenic effects;detergents;The Ames Salmonella/microsome |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 09 Jun 2010 13:27 |
| Last Modified: | 12 Apr 2019 11:18 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/62 |
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