Yaralı, Kaan (2015) A study of the effect of corticosteroids on the differentiation and growth of the adipocytes derived from mesenchymal stem cells. Other thesis, TED Ankara Koleji.
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Abstract
The purpose of my extended essay is to investigate the effect of corticosteroids (CS) on the differentiation and growth of adipocytes from mesenchymal stem cells (MSCs). My research question was “Do CSs have any impact on the differentiation and growth of adipocytes from MSCs?” Six wells were used; Well 1-only MSC (control); Well 2-0.2 mg dexamethasone (DXM) with MSC; Well 3- 5 mg DXM with MSC; Well 4- Adipogenic differentiation solution (ADS) with MSC (ADS control); Well 5- 0.2 mg DXM with ADS and MSC; Well 6- 5 mg DXM with ADS and MSC. Adipogenic differentiation and growth were assessed by an inverted phase-contrast microscope by noting the appearance of fat cells and making cell counts at the 5th, 10th and 15th days. At the 5th day, small fat vacuoles that indicated adipogenic differentiation was noted only in Well 6. At the 10th day, such differentiation was noted in Wells 3, 4, 5 as well as in Well 6 with the maximum cell count in Well 6 (Well 6 versus Wells 1, 2 and 3, p<0.05). The size of the lipid droplets in Well 6 increased when compared to Day 5 assessment. At the 15th day, the growth and differentiation further increased in Wells 4, 5 and 6; Wells 1, 2 and 3 had significantly less cell counts when compared to Wells 4, 5 and 6 (p<0.05). Moreover, at Day 15, the sizes of the lipid droplets in Wells 4, 5, 6 and also Well 3 increased when compared to Day 10 assessment. I conclude that DXM, in a dose-response manner, affect both the development (hyperplasia) and the size (hypertrophia) of adipocytes in the MSC model. These data contribute to our understanding of development of obesity as a side effect of CS treatment.
| Item Type: | Thesis (Other) |
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| Additional Information: | Supervisor: Sevim Saral, IB Notu: A |
| Uncontrolled Keywords: | corticosteroids, mesenchymal stem cells |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 26 Aug 2015 07:49 |
| Last Modified: | 26 Aug 2015 07:49 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/652 |
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