Saltaş, Arda (2015) Investigating the effects of different doses of resveratrol(10µM, 100µM, 1000µM) on the change in the number of the rat mesenchymal stem cells exposed to 25 µM of cisplatin, measured by the real time cell analyzer over 48 hours period. Other thesis, TED Ankara Koleji.
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Abstract
Antioxidants are molecules that inhibit the damaging effects of free radicals which are highly reactive chemicals that are produced when the weak bonds split in a molecule. Cisplatin, on the other hand, is a highly cytotoxic chemotherapeutic agent nearly used for 30 years as an anticancer drug. It inhibits the cell proliferation due to its molecular structure. The purpose of this extended essay is to evaluate the effects of resveratrol, an antioxidant mainly produced by plants such as red grape and Japanese knotweed, to the cytotoxicity caused by cisplatin by using rat (Rattus norvegicus) mesenchymal stem cells. A controlled experiment was carried out with rat mesenchymal stem cells in which the resveratrol concentration was the independent variable and the number of the stem cells given 25µM of cisplatin was the dependent variable. A possible change in the number of the cells would be the indicator of ant cytotoxic effect of resveratrol. Therefore the research question is “How does the number of mesenchymal rat stem cells exposed 25 µM of cisplatin change in different resveratrol concentrations (10µM, 100µM and 1000µM) measured by the real time cell analyzer over 48 hours period?” The hypothesis states that the cytotoxic effect of cisplatin which is a chemotherapeutic agent used in cancer treatments can be reversed by adding different concentrations of resveratrol. The results indicated that moderate doses of resveratrol, approximately 10µM, decreases cytotoxicity due to cisplatin and results in higher rates of reproduction. As seen in the graph, high doses (100µM and 1000µM), on the other hand, have a significant short term effect causing cell index to increase at first. However, their overall effects are not as effective as 10µM dose.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | Supervisor: Şirin Güntürkün, IB Notu: C |
| Uncontrolled Keywords: | Antioxidants, effects of resveratrol |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 20 Aug 2015 07:25 |
| Last Modified: | 20 Aug 2015 07:25 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/574 |
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