The Cytotoxic Effects of Wheat Germ Extract on Glioblastoma Cell Line

Uğur, Yağmur (2014) The Cytotoxic Effects of Wheat Germ Extract on Glioblastoma Cell Line. Other thesis, TED ANKARA KOLEJİ.

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The aim of this experiment was to determine whether wheat germ extract has an increasing cytotoxic activity or any cytotoxic activity on the malignant glioblastoma cancer cells. Thus, this paper includes the comparison of cell viability after the exposition to different concentrations of Wheat Germ Extract (WGE) in medium. My research question was: “How does the cytotoxic activity measured by the percentage of remaining living cells found in Methylthiazol Tetrazolium Assay test (MTT) of glioblastoma multiforme cells differ according to their exposition to different concentrations of wheat germ extract?” My hypothesis was: “As the concentration of applied WGE increases, the cytotoxic activity in glioblastoma cells will also increase. It is expected that the rapidly proliferating cells will be slowed down and largely killed as the WGE concentration increases.” To answer the research question, WGE was produced and given to glioblastoma cells in 6 different concentrations starting from 10% to 35%, increasing 5 by 5 in growth medium. Distilled water was given to the control group at the same concentrations in medium as distilled water was used during the production of WGE and it was important to check whether WGE or distilled water had the cytotoxic effect on cells. The cells were left in a CO2 incubator for 48 hours. After 48 hours, the medium, distilled water, WGE were extracted from the cells and were replaced with MTT. An MTT test was applied and viability was calculated with the results. It was clear that the cytotoxic activity increased as the WGE concentration increased and more than half of the cells went through apoptosis at 35% WGE exposure. Such a conclusion gives hope towards the future of curing of cancer and raises new questions about the usage of WGE.

Item Type: Thesis (Other)
Additional Information: Supervisor: Leyla YAĞUŞ UNGAN, IB Notu: A
Uncontrolled Keywords: Cytotoxic Effects, Glioblastoma Cell, Wheat Germ Extract, WGE
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Kamil Çömlekçi
Date Deposited: 14 Sep 2014 11:55
Last Modified: 14 Sep 2014 11:55

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