Taşdelen, Umut (2014) Coculturing of Rat Islet Cells with Human Amnion Membrane to Increase Their Cell Viability and Production of Insulin. Other thesis, TED ANKARA KOLEJİ.
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Abstract
In nowadays, Diabetes Mellitus is a growing threat to humans’ health. Unfortunately, there is no permanent and effective cure for it. Usually, transplantations of Langerhans islets or pancreases are not successful in long terms because of immune sytems attack on them. The aim of this research is to find out the potential of usage of human amnion membrane as a protective membrane against the immune system in islet transplantations. No research similar to this one has been done which means that this study is the first one in literature. My research question is “How does human amnion membrane affect rat langerhans islet’s cell viability and functionability during coculture by fluorescent microscopy and glucose stimulation test?”. The HAM (Human Amnion Membrane) is the inner layer of the placenta that surrounds the baby during pregnancy. HAM shares its cellular origin with the fetus; together they grow in parallel throughout pregnancy. It supports cell growth and repairing processes. It is cheap and because of its extensive usage in wound clothing, especially in eye wound clothing, it is easy to find. For these reasons, HAM is a good candidate for be used as a protective membrane in langerhans islet transplantations. During the experiment, 3 experiment group and one control group was taken to coculture with HAM and HAM position in respect to the islets was different in each group. Before the culture, cell viability and insulin indexes of experiment groups were measured by fluorescent microscopy and glucose stimulation test. After 48 hours in coculture, cell viability and glucose stimulation tests were done again to measure the final cell viability and insulin indexes of experiment groups. Inıitial cell viability and insulin indexes are 81.0% and 3.4 respectively G2 (has no insert solution between islets and HAM) has the lowest final mean cell viability (%64) and index of insulin (2.2) in the experiment groups. Control group’s (only islets) final index of insulin and mean cell viability are 3.7 and %82 respectively. G3 (islets at the top, HAM at the bottom, includes insert) has the second highest final mean cell viability (%86) and index of insulin (4.2). G1 has the highest final mean cell viability (%93) and index of insulin (5.8). Standard error of control group’s final cell viability is 0.63, standard error of final cell viability of G1 is 0.90, G2 ‘s standard error of final cell viability is 1.23 and standard error of final cell viability of G3 is 0.63. Standard error of control group’s final insulin index is 0.36, standard error of insulin index of G1 is 0.33, G2 ‘s standard error of final insulin index is 0.14 and standard error of final cell viability of G3 is 0.06.P value of one way ANOVA test of cell viability and glucose stimulation test are (p= 4.73 X 10-12, p= 1.19 X 10-16 ) respectively. These p values are smaller that alpha value which means HAM can be used as a protective membrane in Langerhans islet transplantations.
| Item Type: | Thesis (Other) |
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| Additional Information: | Supervisor: Demet İzgü |
| Uncontrolled Keywords: | Rat Islet Cells, Human Amnion Membrane, Insulin |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 12 Sep 2014 12:47 |
| Last Modified: | 12 Sep 2014 12:47 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/557 |
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