Nazlıel, Kerem (2014) Is there a significant mean difference among antiseptics used in medical institutions, in terms of their bactericidal effects on Staphylococous epidermidis in laboratory conditions evaluated by the utilization of the Quantitative Suspension Test Method? Other thesis, TED ANKARA KOLEJİ.
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Abstract
The aim of this extended essay was to evaluate the bactericidal effects of different brands of antiseptics on the bacterium Staphylococcus epidermidis. My research question was: “Is there a significant mean difference among antiseptics used in medical institutions, in terms of their bactericidal effects on Staphylococous epidermidis in laboratory conditions evaluated by the use of Quantitative Suspension Test Method?” It was hypothesized that there would be a mean difference in between antiseptic properties of various agents. Since the antiseptics include different chemicals, they may have different bactericidal properties in terms of destroying microorganisms. In order to test the hypothesis and to answer the research question, Quantiative Suspension Test Method was used. S. epidermidis colonies were cultivated onto Mueller Hinton Plate. In order to compare the data, a control trial was needed. In my research, S. epidermidis suspensions were prepared, by using dilutions and then mixed with different brands of antiseptics. After 60 seconds of preparation the solution was neutralized. Then the suspensions were spreaded on a TSA and incubated for 24 hours at 37°C. Following the incubation the colonies were taken out and counted by using a colored marker and multiplied by the dilution ratio in order to determine the actual number. The results achieved turned out to prove that the hypothesis was right. The p value was 0.042 on single factor ANOVA. This value shows that there was a mean difference among the bactericidal effects of antiseptics. Monorapid turned out to be the most effective one while Aniosrub was the least. My study demonstrated that, different antiseptics have different bactericidal effects. It is possible that with time, bacteria may gain resistance to commercial antiseptics. I believe in order to prevent the spread of these infections especially in hospital settings it is mandatory to test the antiseptic properties of different antiseptics in regular time intervals.
| Item Type: | Thesis (Other) |
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| Additional Information: | Tutor: Demet İzgü, IB Notu: B |
| Uncontrolled Keywords: | antiseptics, bactericidal effects, Staphylococous |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 12 Sep 2014 07:04 |
| Last Modified: | 12 Sep 2014 07:04 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/529 |
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