Taş, Alper (2014) Investigating the cytotoxic effect of milk powder on U‐118 MG human glioblastoma cell series. Other thesis, TED ANKARA KOLEJİ.
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Abstract
The purpose of my extended essay is to observe the cytotoxic effect of milk on the human glioblastoma cells. In order to reach this purpose, different concentrations of milk powder solutions are prepared and the inverse proportion between the concentration of the milk powder solutions and the viability of the cells is shown. The research question is “Does milk powder cause a cytotoxic effect on U‐118 MG Human Glioblastoma Cells; does the cytotoxic effect that milk powder causes on human glioblastoma cells increase as the concentration of the milk powder solution increases?” It is hypothesized that; there will be a significant mean difference between the number of human glioblastoma cells when the concentration of milk powder solution is increased. In order to search the validity of the hypothesis, U‐118 MG Human Glioblastoma Cell Series are used. These cells are resolved in a medium with 90% RPMI with L‐glutamine, %10 Fetal Bovine Serum and 1% antibiotics. For the passaging procedure Trypsin‐EDTA solution is used. By using the counting chamber cell concentrations which contains 20.000 cells in 200 μL of medium is prepared. Then milk powder solutions with the concentrations of 10, 20, 40, 80 100 mg/ml and a control group in which no milk powder solution is added are prepared. Five trails are made for each group and by using MTT Assay, cell viability is measured for the 24th hour. Statistical analyse is done to show the decrease in the viability of the cells. The data collected with the MTT assay is processed and the mean of the trials for each group is calculated. By using t‐Test, the significant decrease in the viability of the cells is found. Although the decrease for the concentration 10 mg/ml solution of milk powder isn’t significant, the decrease for the concentrations of 20, 40, 80 and 100 mg/ml solutions of milk powder is significant since the p values for them are smaller than 0.05. By using Anova Statistics, it is seen that there is a significant mean difference between the absorbance values of solutions which contain different concentration of milk powder. As a result of the experiment, it is found out that the milk powder has a cytotoxic effect on the glioblastoma cells and the number of the cancer cells decrease as the concentration of the milk powder solution is increased.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | Supervisor’s Name: Sevim Saral, IB Notu: B |
| Uncontrolled Keywords: | cytotoxic effect, milk powder, human glioblastoma cell |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Users 114 not found. |
| Date Deposited: | 09 Sep 2014 10:18 |
| Last Modified: | 09 Sep 2014 10:18 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/457 |
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