Dursun, Merve (2013) Comparison of destabilizing activities of a well-known αβ-fibril destabilizer rifampicin with methylene blue for its usage in Alzheimer’s disease treatment. Other thesis, TED ANKARA COLLEGE FOUNDATION PRIVATE HIGH SCHOOL.
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Abstract
The aim of this experiment was to determine whether methylene blue has a destabilizing effect on αβ-fibrils or not for the usage in Alzheimer’s disease treatment. So that this extended essay includes the comparison of the destabilizing effect of methylene blue with rifampicin which is a well-known αβ-fibril destabilizer. My research question was “Does the destabilizing effect of methylene blue on Aβ-fibril formation in vitro sufficient for its usage in the Alzheimer’s disease’s treatment compared to Rifampicin?” My hypothesis was “Methylene blue can destabilize Aβ-fibril formation which is a symptom of Alzheimer’s disease in vitro as much as Rifampicin.” In order to answer the research question and test the hypothesis, Ellman’s spectrophotometric method and thioflavin-t fluorometric method was used. Ellman’s spectrophotometric method was to calculate the IC50 value of methylene blue which will be used in the second part. Second part was the comparison of the fluorescence concentrations at the end of the 24th hour. Destabilizers at their IC50 values were put into bathtubs and the bathtubs were put into bacteriological incubator. At the end of the 24th hour tubes were put into fluorometer device and the fluorescence concentrations were measured. As fluorescence concentration is directly proportional with αβ-fibril formation this measurement gave the necessary data about the destabilizing activity of methylene blue and rifampicin. At the end of the 24th hour fluorescence concentrations in the tubes that contain methylene blue and rifampicin were close to each but t-test results showed that there is difference between them. After the calculation of percentage effects of the destabilizer, the difference was more observable: the effect of methylene blue was more compared to rifampicin. This gives hope about the treatment of Alzheimer’s disease but also brings new questions to investigate.
| Item Type: | Thesis (Other) |
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| Additional Information: | Supervisor: Fuat İsmet ŞİŞMAN, IB Notu: A |
| Uncontrolled Keywords: | methylene blue, Alzheimer, αβ-fibril destabilizer |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Users 114 not found. |
| Date Deposited: | 18 Sep 2013 06:18 |
| Last Modified: | 18 Sep 2013 06:18 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/384 |
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