Coşkuntürk, Yiğitcan (2012) Comparison of the Probiotic Effects of Lactobacillus acidophilus and Enterecoccus faecalis as indicated by autoaggregation and coaggregation percentages. Other thesis, TED Ankara College Foundation High School.
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Abstract
The aim of this extended essay was to compare the probiotic penetrances of Lactobacillus aciophilus and Enterecoccus faecalis which is indicated by percentages of autoaggregation and coaggregation. The research question was “How do the probiotic effects of Lactobacillus acdiophilus and Enterecoccus faecalis differ from each other as indicated by autoaggregation and coaggregation percentages?”. It was hypothesized that “L.acidophilus will have higher percentages of autoaggregation and coaggregation, which shows that L.acidophilus has higher probiotic effect compared to E.faecalis.” To test this hypothesis, a common procedure, Vandervoorde et. al. (1992) was used with some modifications to conduct the aggregation and coaggregation assays. For the coaggregation assay E.coli is needed besides L.acidophilus and E.faecalis as E.coli is prime for the human gut flora. The autoaggregation and coaggregation results were calculated by a formula using optical densities measured by spectrophotometer. The average percentage of autoaggregation for L.acidophilus is 82 % and average percentage of coaggregation for E.faecalis is 20.2 %. When average percentage of coaggregation is taken into consideration it is seen that coaggregation percentage of L.acidophilus is higher than E.faecalis. Average percentage of coaggregation for L.acidophilus is 65 % and for E.faecalis it is 7.5 %. The statistical analysis was done by using one-tailed t-test. The p values were found to be 2,55119E-10 for autoaggregation and 2,51E-11 for coaggregation. Since both p values were below 0.05, the hyptohesis was supported. L.acidophilus has higher percentages of autoaggregation and coaggregation and this results indicate that L.acidophilus has more probiotic penetrance than E.faecalis since percentages of autoaggregation and coaggregation are criteria for selecting probiotic bacteria.
| Item Type: | Thesis (Other) |
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| Additional Information: | Supervisor: Meltem Başer, IB Notu: C |
| Uncontrolled Keywords: | probiotic penetrances, Lactobacillus aciophilus, Enterecoccus faecalis, probiotic effects |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 10 Jul 2012 07:00 |
| Last Modified: | 12 Apr 2019 09:24 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/238 |
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