How much time is needed to sterile objects from Escherichia coli DH 5 alpha with Ultraviolet light exposure?

Han, Öykü (2012) How much time is needed to sterile objects from Escherichia coli DH 5 alpha with Ultraviolet light exposure? Other thesis, TED Ankara College Foundation High School.

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In our daily lives, we sterilize lots of tools like manicure tools and aquarium equipments. Sterilization with Ultraviolet light is a way of getting rid of pathogenic organisms among other ways. By UV sterilization, the nucleic acids of the pathogenic organism’s genetic material are damaged. This damage prevents the replication of the organisms and in some cases the UV light also kills the organisms. But the organisms should be under the UV light for a certain time interval for the damage to be done. This time interval changes from organism to organism. In this paper the time interval needed for the tools to be sterile from the pathogenic bacteria, Escherichia coli DH alpha 5, is investigated. Because E.coli is a type of bacteria, that is capable of living in both the tools that are sterilized with UV light and the humans. Therefore, the bacteria can be transmitted to the humans by those tools and they can cause illnesses. So it is important for us to use sterile tools to avoid illnesses. The experiment that is explained in this paper searches the time limit for when the tools we use are sterile for use. It was found that the tools that contain E.coli bacteria should be exposed to the Ultraviolet light for at the very least 60 minutes. But even 60 minutes of UV light exposure isn’t sufficient for sterilization because there are still bacteria remaining after the exposure. If the bacteria are left under the UV light below 60 minutes, the tools aren’t considered sterile and it is recommended that tools containing E.coli after UV exposure shouldn’t be used.

Item Type: Thesis (Other)
Additional Information: Supervisor: Vidad Elemin Şemşir, IB Notu: C
Uncontrolled Keywords: Sterilization, Ultraviolet light
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Kamil Çömlekçi
Date Deposited: 09 Jul 2012 12:28
Last Modified: 12 Apr 2019 09:38

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