Investigating the antibacterial activities of methanol, water and hexane extracts of Hypericum bityhnicum on Streptococcus pyogenes.

Şahinöz, Melis (2012) Investigating the antibacterial activities of methanol, water and hexane extracts of Hypericum bityhnicum on Streptococcus pyogenes. Other thesis, TED Ankara College Foundation High School.

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In the present study methanol (-MeOH), water (-H2O), Hexane (Hx) extracts of H. bithynicum, are screened for their in vitro antibacterial activity. The research question of the experiment is: “Is there a statistically significant difference among the Minimal Inhibitory Concentration (MIC) values of methanol, hexane and water extracts of H. bithynicum with regards to values on Streptococcus pyogenes in laboratory conditions?” In this respect, the aim of this investigation is to scrutinize the bactericidal properties of the extract of Hypericum Bithynicum plant prepared with methanol, water and hexane solvents on the bacterium. The hypothesis is that; there would be a statistically significant difference with respect to obtained Minimal Inhibitory Concentration values between the groups as; hexane and water extracts will bear greater antibacterial activity towards S. pyogenes when compared to methanol extract of H. bithynicum. In order to examine the hypothesis and to find an answer to the research question, the Broth Dilution Method was used. For growing and diluting the bacterial suspensions, Mueller Hinton Broth (MHB; Difco) and Mueller Hinton Agar (MHA; Oxoid) were assigned, along with the utilization of microdilution method. After incubation of impregnated bacteria and extracts for 18 hours, the lowest concentration of the extracts that completely inhibited the macroscopic growth of bacteria (MIC) was indicated. The data obtained showed that average MIC values of methanol extract is 63.28 µg/ml, hexane extract is 32.20 µg/ml and water extract is 30.06 µg/ml Consequentially, it was revealed that the antibacterial activity of water extract is stronger than that of hexane which displayed more effective antibacterial properties when compared to the methanol extract (since the group that had the lowest MIC values had greater antibacterial activity). ANOVA results were parallel with the hypothesis of the experiment as, the difference between the extracts methanol and water was not statistically significant; whereas the pairs: methanol-water and methanol-hexane displayed significant mean difference in terms of their MIC values.

Item Type: Thesis (Other)
Additional Information: Supervisor: Meltem BAŞER, IB Notu: C
Uncontrolled Keywords: methanol (-MeOH), water (-H2O), Hexane (Hx), bithynicum, antibacterial activity
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Kamil Çömlekçi
Date Deposited: 09 Jul 2012 12:15
Last Modified: 12 Apr 2019 10:00

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