Cengiz, Göksu (2011) Investigating the mass of Soya Bean Kazein which has the same content of protein with 2,7g Tryptose Phosphate Broth. Other thesis, TED ANKARA COLLEGE FOUNDATION HIGH SCHOOL.
![[img]](http://tedprints.tedankara.k12.tr/style/images/fileicons/application_pdf.png)
|
PDF
goksu_cengiz.pdf Available under License Creative Commons Attribution Non-commercial. Download (633kB) |
Abstract
Cell cultures are used rather frequently due to its being economic, easy to control and allowing industrial products to yield. The most common area of usage of cell culture is the vaccine production and vaccine of Foot and Mouth Disease is the one of the firsts in this group. The method of vaccine production for the Foot and Mouth Disease is using suspensionof BHK-21(Baby Hamster Kidney) cells with the Glasgow Media. In this media there are two main elements, the cells and the nutrients for the source of energy. As a sorce of nutrient 2,7g Tryptose Phosphate Broth (TPB) is used per liter of a Glasgow media. However TPB is expensive and have some contaminant affects so another vegetal pepton Soya Bean Kazein(TSB) is tried instead of TPB in this experiment. Therefore, this study is searching an answer to the question : “How much Soya bean kazein should be used per liter in order to obtain the same absorbance value on the spectrophotometer with 2.7 g/l tryptose phosphate broth sample which is the standart value used in Glasgow media as an energy source for growth of cells in the vacines of Footh an Mouth Diseases Virus?.” By using Lowry method and a spectrophotometer the mass of protein in 2,7 g TPB and 0,5-1,0-1,5-2,0-2,5 and 3,0 g TSB was recorded. It was found that 3,0 g TSB can be used in one liter of Glasgow media instead of 2,7 g TPB.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | Supervisor: Ümit Yaşatürk Midilli IB Notu: A |
| Uncontrolled Keywords: | Soya Bean Kazein, Biology, Cell cultures, vaccine production, Tryptose Phosphate Broth |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Kamil Çömlekçi |
| Date Deposited: | 22 Jun 2011 06:26 |
| Last Modified: | 12 Apr 2019 10:37 |
| URI: | http://tedprints.tedankara.k12.tr/id/eprint/154 |
Actions (login required)
 |
View Item |
